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p-pak3 (thr421  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc p-pak3 (thr421
    P Pak3 (Thr421, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p-pak3+(thr421/anti+pak3/10__1523_slash_jneurosci__4816___10__2011-108-194-215
    Average 90 stars, based on 1 article reviews
    p-pak3 (thr421 - by Bioz Stars, 2026-09
    90/100 stars

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    Article Title: Aberrant Calcium/Calmodulin-Dependent Protein Kinase II (CaMKII) Activity Is Associated with Abnormal Dendritic Spine Morphology in the ATRX Mutant Mouse Brain
    Article Snippet: Mouse brains were immediately removed and perfused in ice-cold buffer for 3 min (0.32 m sucrose, 20 m m Tris-HCl, pH 7.4). mPFCs were dissected and then homogenized in 200 μl of buffer containing 50 m m Tris-HCl, pH 7.4, 0.5% Triton X-100, 0.5 m NaCl, 4 m m EDTA, 4 m m EGTA, 1 m m Na 3 VO 4 , 50 m m NaF, 1 m m DTT, 2 μg/ml pepstatin A, 1 μg/ml leupeptin, and 100 n m calyculin A, and then treated with Laemmli's sample solution and boiled for 3 min. Antibodies included rabbit polyclonal antibodies against p- CaMKII (α-Thr286/β-Thr287) (1:5000) ( Fukunaga et al., 1988 ); CaMKII (1:5000) ( Fukunaga et al., 1988 ); synapsin I (1:1000) ( Fukunaga et al., 1992 ); protein phosphatase 1 (PP1) (1:1000, the catalytic subunit of PP1γ-1 and -2) ( Fukunaga et al., 2000 ); PP2A catalytic subunit (1:1000) ( Fukunaga et al., 2000 ); calcineurin (1:1000) ( Shioda et al., 2006 ); p- CaMKI (Thr177), CaMKI ( Han et al., 2009 ); p- CaMKIV (Thr196), CaMKIV ( Kasahara et al., 1999 ), NR1, kalirin, p21-activated kinase 3 (PAK3), spinophilin, glutamate receptor 1 (GluR1), p- Ser/Thr, p -protein kinase Cα (PKCα) (Ser657), PKCα (all at 1:1000, Millipore); Tiam1 (1:1000, Calbiochem); ATRX (H-300, 1:500, Santa Cruz Biotechnology); and p- PAK1 (Thr423), p- PAK2 (Thr402), p- PAK3 (Thr421), PAK1, PAK2, myosin II regulatory light chain (MLC), p- MLC (Ser19), extracellular signal-regulated kinase (ERK), p- ERK (Thr202/Tyr204) (all at 1:1000, Cell Signaling Technology).

    Article Title: Aberrant Calcium/Calmodulin-Dependent Protein Kinase II (CaMKII) Activity Is Associated with Abnormal Dendritic Spine Morphology in the ATRX Mutant Mouse Brain
    Article Snippet: Mouse brains were immediately removed and perfused in ice-cold buffer for 3 min (0.32 M sucrose, 20 mM TrisHCl, pH 7.4). mPFCs were dissected and then homogenized in 200 l of buffer containing 50 mM Tris-HCl, pH 7.4, 0.5% Triton X-100, 0.5 M NaCl, 4 mM EDTA, 4 mM EGTA, 1 mM Na3VO4, 50 mM NaF, 1 mM DTT, 2 g/ml pepstatin A, 1 g/ml leupeptin, and 100 nM calyculin A, and then treated with Laemmli’s sample solution and boiled for 3 min. Antibodies included rabbit polyclonal antibodies against p-CaMKII ( -Thr286/ - Thr287) (1:5000) (Fukunaga et al., 1988); CaMKII (1:5000) (Fukunaga et al., 1988); synapsin I (1:1000) (Fukunaga et al., 1992); protein phosphatase 1 (PP1) (1:1000, the catalytic subunit of PP1 -1 and -2) (Fukunaga et al., 2000); PP2A catalytic subunit (1:1000) (Fukunaga et al., 2000); calcineurin (1:1000) (Shioda et al., 2006); p-CaMKI (Thr177), CaMKI (Han et al., 2009); p-CaMKIV (Thr196), CaMKIV (Kasahara et al., 1999), NR1, kalirin, p21-activated kinase 3 (PAK3), spinophilin, glutamate receptor 1 (GluR1), p-Ser/Thr, p-protein kinase C (PKC ) (Ser657), PKC (all at 1:1000, Millipore); Tiam1 (1:1000, Calbiochem); ATRX (H-300, 1:500, Santa Cruz Biotechnology); and p-PAK1 (Thr423), p-PAK2 (Thr402), p-PAK3 (Thr421), PAK1, PAK2, myosin II regulatory light chain (MLC), p-MLC (Ser19), extracellular signal-regulated kinase (ERK), p-ERK (Thr202/Tyr204) (all at 1:1000, Cell Signaling Technology).



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